Morphine - properties and testing from THE NATIONAL FORMULARY, 8TH EDITION (1946) Description: Morphine HCl occurs as white, silky, glistening needles or cubical masses, or as a white, crystaline powder. It is odorless, has a bitter [MOST alkaloids have bitter tastes...], and is permanent in the aid but is affected by light (???) Soluability: 1G of Morphine HCl dissolves in about 17.5cc of water and in about 52cc of alcohol at 25c; also in about .5cc of boiling water or 46cc of alcohol at 60c. It is soluable in glycerin, but insoluable in CHCl3 and ether. Identification: A: An aqeuous solution of Morphine HCl responds to the tests for chloride ion [well, duh!?!?] B: Place about 5mg of Morphine HCl on a watch glass, add .5cc of diluted sulfuric acid and heat on a water bath for about 5 minutes. Cool and add 5cc of sulfuric acid, containing in each cc 5mg of selenous acid: an intense blue color appears which slowly changes to green and finally to brown. C: Morphine HCl with sulfuric acid, containing 5mg molybdic acid in each cc, produces a purple color, changing to blue. D: Sulfuric acid, containing in each cc. 1 drop of formaldehyde T.S., yields an intensely purple color with Morphine HCl. E: With nitric acid Morphine HCl produces an orange-red color fading to yellow. F: The addition of a few drops of freshly prepared ferric chloride T.S. to an aqueous solution of Morphine HCl (1 in 100) produces a blue color, which is destroyed by acids, by alcohol, or by heating. G: Add potassium ferricyanide T.S. containing 1 drop of ferric chloride T.S. in each cc., to an aqeuous solution of Morphine HCl (1 in 100): a deep blue color is produced at once (***DIFFERENT FROM CODEINE!!!***) There should be no residue after ignition of Morphine HCl When a solution of Morphine HCl is warmed with NaOH, there should be no evidence of the odor of ammonia. (tests for) Apomorphine: Add .1gm sodium bicarbonate and .1cc of iodine T.S. to an aqeuous solution of Morphine HCl (1 in 50): when shaken with an equal volume of ether, both layers should remain free from even a transient green color. Meconate: Add a few drops of ferric chloride T.S. to 5cc of an aqueous solution of Morphine HCl (1 in 30), previously mixed with 5cc of diluted hydrochloric acid: no red color is produced. (removal) Foreign Alkaloids: Disolve 1 gram of Morphine HCl in 10cc of NaOH T.S. in a seperator, and shake the solution with successive portions of 15cc, 10cc, and 10cc of chloroform, passing the chloroform solutions through a small filter previously moistened with chloroform. Shake the combined chloroform solutions with 5cc of distilled water, seperate the chloroform, and evaporate it carefully to dryness on a water bath [uh, avoid the nasty carcinogenic fumes which will also knock you out while your at it too] Add to the RESIDUE thus obtained 10cc of .002 N sulfuric acid, heat gently until disolved, cool, add 2 drops methyl red T.S., and titrate the excess H2SO4 with .02 N NaOH: not less than 7.5cc of the latter should be required. -- ____ ______ ________ _____ / \ | \| /\ | \ jkenner@cello.gina.calstate.edu / \| _ \ \/ | _ \ /___/\ \___|> > |__|> > BORN TO BE WIRED... / | / /\ | / All the sugar and twice the \_________|______/|___\/__|______/ caffeine of regular netusers! finger me and make a pgp key come.